Submitted by nia4019 on February 5, 2024 - 2:16pm
| Title | Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy. |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Liu H, Qin W, Shao Y, Ma Z, Ye T, Borg T, Gao BZ |
| Journal | J Biomed Opt |
| Volume | 16 |
| Issue | 12 |
| Pagination | 126012 |
| Date Published | 2011 Dec |
| ISSN | 1560-2281 |
| Keywords | Animals, Animals, Newborn, Cells, Cultured, Equipment Design, Microscopy, Fluorescence, Multiphoton, Muscle Development, Myocytes, Cardiac, Rats, Sarcomeres |
| Abstract | We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis. |
| DOI | 10.1117/1.3662457 |
| Alternate Journal | J Biomed Opt |
| PubMed ID | 22191929 |
| PubMed Central ID | PMC3245745 |
| Grant List | K25 HL088262 / HL / NHLBI NIH HHS / United States P20 RR021949 / RR / NCRR NIH HHS / United States P20RR0 949 / RR / NCRR NIH HHS / United States 1K25HL088262-01 / HL / NHLBI NIH HHS / United States |